Top-Notch DNA Isolation Lab Report Writing Assistance
A complete copy of DNA is found in all cells both human and microorganisms. At Custom writing bay, we are a group of skilled DNA structure and function experiment writers dedicated to providing you with credible and up to standard laboratory work. Our DNA structure and function experiment writers are well versed in the process and procedures involved in the DNA isolation process. At www.customwritingbay.com, we understand that for DNA to be isolated, scientists must first break open the cells and remove structural proteins and enzymes that may interfere with the DNA structure. In eukaryotes, the DNA is contained in the membrane wrapped nucleus whereas in prokaryotes the DNA is found floating free in the cytoplasm. In a learning institution, you will find different learning departments whereby scholar’s involvement is highly required in order to gain knowledge.
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Our DNA structure & function experiment writers are aware that this is the reason why most of the isolation experiments choose prokaryotes as a source of DNA. In offering DNA isolation lab report writing help, our writers are familiar with the various reagents that are used in DNA isolation and the roles they play in isolation of DNA. These are the major ingredients used in DNA extraction namely, non-iodized table salt, dishwashing detergent, and ice cold ethanol in a test tube. Soap and salt are useful in disruption of the cell wall and membranes and hence the disentanglement of the DNA. DNA structure & function experiment writers at www.customwritingbay.com know that heat also serves to speed up the reaction. Use of enzymes such as papain is important as they serve to breakdown DNAse the enzyme that breaks down DNA. Cold ethanol precipitates the DNA making it spoilable. Our DNA isolation lab report writing Help service is familiar with the protocol that is supposed to be followed when writing up a good laboratory report. This includes the various subdivisions in a laboratory report, such as the introduction where a hypothesis or a principle that is already in existence is to be investigated, the abstract, materials, and procedures followed in the experiment, then the results section that caters for the data of the experiment represented in tables and graphs. At Custom writing bay, our DNA structure & function experiment writers finalize a laboratory report with a conclusion which discusses whether the experiment was a success or not based on the hypothesis that was set. In our DNA isolation lab report writing help, we have identified the isolation of DNA as one of the basic procedures in molecular biology studies, making us the site to visit for all your DNA isolation lab report writing help.
Structure, Isolation, and Function of DNA
Role of the Experiment
The role is to isolate DNA from Onion cells to facilitate the study of its structure and functionality.
The cells were first broken off. In the experiment, 20-30 grams of yellow onion is placed in a mortar and 20g of acid-washed sand added. These are ground vigorously with a pestle for five minutes. A yellow mush forms into 100ml of distilled water heated to 60o 10ml of NaCl and 10ml of detergent are added. The ground onion was added to 10ml of the saline-detergent solution and stirred to dissolve for 5 minutes ensuring that the temperature was maintained at 60o. The suspension was then decanted then filtered leaving cellular debris and sand behind. The solution was then cooled to room temperature. Twice the volume of cold absolute ethanol was added to the viscous solution that contained DNA. Care is taken so that the alcohol flows to the side of the beaker settling on the aqueous solution and forms a layer. A flame sterilized glass rod is then inserted gently into the solution and rotated clockwise once. Rotation spools all the DNA onto the glass rod and it is then transferred to a test tube containing 95% ethanol. The rod and spooled DNA are then dried with a clean filter paper and observed. The DNA strand was probed via the use of diphenylamine which gives a blue color when in contact with deoxyribose.
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