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Gel filtration or gel permeation chromatography is also known as size exclusion chromatography. At Custom writing bay, our professional writers are familiar with the principles and procedures that are involved in size exclusion chromatography. In offering gel lab report writing help, at www.customwritingbay.com, we are aware that the separation of biological molecules is based on the differences in the size and /or shape of the analyte molecules. This governs the analytes access to the pore volume inside the column packing. We also realize that the fractionation range identifies the molecular weight range from the largest molecule that is fully included in the smallest molecule that is excluded.
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Our gel filtration chromatography lab report writers are familiar with the fact that gel filtration chromatography is the simplest and mildest of all chromatography techniques. This technique can be applied in group separations where components of a sample are separated into two major groups according to their size range. Our writers at custom writing bay are aware that this method of separation can be used to remove high or low molecular weight contaminants. On high-resolution fractionation of biomolecules, the components of a sample are separated according to differences in their molecular weights. This method can be used to effectively isolate one or more components, to determine molecular weight distribution analysis and to separate monomers from aggregates. In offering gel lab report writing help, our writers are familiar with the fact that gel filtration is a technique that is well suited to the handling of biomolecules that are sensitive to the changes in pH, the concentration of metal ions, cofactors and harsh environmental conditions.
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According to gel filtration chromatography lab report writers at Custom writing bay, separation is performed in the presence of essential ions, cofactors, urea, detergents, guanidine hydrochloride, at high or low ionic strength at a temperature of 37oc, or in a cold room. To perform a gel filtration, the gel filtration medium is packed into a column to form a packed bed. The porous matrix is chosen due to its inertness and chemical and physical stability. The packed bed is equilibrated with a buffer which fills the pores of the matrix and the space in between particles. Our gel filtration chromatography lab report writers realize that according to their size, smaller analytes have partial to complete access to the volume of the pore. For analytes that partially or fully enter the pore volume, the larger molecules with less access to the pore volume will elute first, while the smallest molecules will elute last.
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- It should demonstrate the ability by the scholar to communicate information in a clear and concise manner.
- A great lab report should be able to present a credible hypothesis and the way to test it.
- The results should consist of raw data that is not interpreted in any way whatsoever.
- A connection between the findings and the background information should be evident in the Lab Report.