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Introduction to Molecular Genetics
Role of the Experiment
In this experiment, the colonies of Streptococcus pneumoniae both R and S were acquired. The S cells were killed by heat and thereafter mice to be used in the experiment were grown. In the first mouse, living S colonies were injected; the second mouse live R colonies were injected. In the third mouse, the heat treated S cells were inoculated. The fourth mouse, living R colonies as well as heat treated S cells were inoculated. Bacteria isolated from the blood samples of each of these mice were cultured and the colonies that were formed observed.
Mice injected with living S cells get pneumonia, while those that are injected with the living R cells or heat treated S cells remain healthy. The mice injected with a mixture of living R cells and heat treated S cells contract the disease and die of pneumonia. Bacteria isolated from the blood samples indicate that the dead mice produced S cultures with a capsule similar to that of the injected S cells, even though the injected S cells had been killed by exposure to heat.