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Amylase is an enzyme that breaks down starch to maltose. In an effort to understand how this enzyme works, it is essential that various experiments be carried out. This necessitates the writing of an amylase activity Lab report. In this case, experiment done should provide a method to measure the amount of maltose produced, determine the effect of enzyme concentration on the rate of reaction, the effect of different concentrations of substrate on the reaction rate, and the effect of temperature as well as the pH on the reaction rate. At custom writing bay we recommend a series of experiments to demonstrate all the factors necessary for amylase enzyme activity. To be able to test maximum amylase activity quantitatively, a series of variables will be manipulated. In one part of the amylase activity lab report, the manipulated variable is the enzyme concentration, whereas the controlled variables are the temperature and the volume of the substrate. In the second part, the enzyme concentration changes while the substrate volume and temperature remains constant. Writers at Custom writing bay argue that the third part manipulates temperature while controlling the enzyme and substrate volume. The fourth part then controls all the factors namely, the substrate concentration, enzyme concentration, and the temperature at which the reaction takes place.
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Thereafter, in the experimental set up done to put together an amylase activity lab report, a spectrophotometer is used to determine the absorbance rate. However, before use, it must be calibrated with a control that is mainly distilled water. For the control of the qualitative results, the iodine solution turns dark blue when dropped into the starch solution. At our site, we understand that in the amylase activity lab report results, if there is iodine present in solution, it turns deep blue if there was any residual or starch that was unconverted. In the case of partially degraded starch, it gives a brown or red color. Yellow represents totally degraded starch. Once a benedicts test is carried out, the solution is blue when there are no sugars present and turns red when there is sugar in the solution. A graph of each of the variants is then plotted for ease of understanding and demonstration. At custom writing bay , we provide you with the assistance with all your amylase activity lab report.
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