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Development of the polymerase chain reaction as a very tool of molecular biology has been improving since 1985. Professional writers at Custom writing bay understand that the PCR demonstrates its power to amplify very small amounts of template nucleic acid as well as to amplify different nucleic acids namely DNA and RNA. The major drawback of PCR is that it is highly susceptible to contamination from its own products. At Custom writing bay, writers who offer PCR lab report writing help are well versed in the process of PCR. First, human genomic DNA suitable for PCR analysis is obtained from mainly buccal(cheek) cells because their collection is rapid, non-invasive, painless as well as bloodless. Our PCR and gel electrophoresis lab report writers are aware that the cell lysate obtained in boiling cheek cells is extremely crude and contains target DNA as well as other molecules that inhibit the activity of the Taq DNA polymerase. Chelex, a negatively charged resin is employed to bind positively-charged metal ions present in the cell lysate. The metal ions are known to be inhibitors of the Taq DNA polymerase.
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A sample of the supernatant, which contains chromosomal DNA is then combined with a buffered solution of heat stable Taq polymerase, short oligonucleotide polymers, and the four deoxynucleotide building blocks of DNA and finally the cofactor MgCl2. The PCR mixture is then placed in a DNA thermal recycler and then taken through the 30 cycles. Our PCR and gel electrophoresis lab report entails identifying the various steps involved in PCR. In the first step, the mixture is exposed to heat for 1 minute at 94oC. In this step, the chromosomal DNA is denatured into single strands. The next step is a minute at 65oC, where after this step, the primers form hydrogen bonds to their complementary sequences on either side of the locus. The final step involves exposure to a temperature of 72oC for one minute, where Taq DNA polymerase extends a complementary DNA strand from each primer.
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The DNA is attained with ethidium bromide for ease of visualization. We are aware that different alleles will appear as different bands with each band position indicating the size, where a smaller band moves a longer distance from the origin and a larger one moves a shorter distance.
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- Ensure that your report is complete and well organized by following a clearly spelled out format by your institution.
- The introduction of your Lab Report should have a summary of the literature review and state how the experiment will add to the knowledge already in existence.
- All the important parts of the methodology such as the instruments for measurement and techniques should be clearly stated.
- The results should contain a summary of the data obtained from the experiment. The data collected should not be interpreted.